Can I unmask tumor
heterogeneity cell by cell?

YES! Contact Me YES! Request Pricing

Decipher Tumor Heterogeneity and Complexity
with Single Cell Sequencing

Cancer is a dynamic disease characterized by genomic instability, increasing both genetic and epigenetic tumor heterogeneity as the disease develops. The microenvironment surrounding the tumor adds an additional layer of complexity—this mixture of cell populations includes tumor subclones, immune and inflammatory cells, vascular networks, and stromal cells.

10x Genomics offers single cell sequencing approaches that allow you to get a detailed understanding of these diverse cell types, and the interactions between them, to impact how cancers are diagnosed and treated.

  • Characterize hundreds to tens of thousands of cancer cells, without the need to pre-select cell types or markers
  • Analyze gene and protein expression, genotypes, and gene regulation on a cell-by-cell basis
  • Identify novel targets and discover new cancer biomarkers
  • Dissect the genetic and epigenetic pathways driving malignancy and tumor progression
  • Map clonal evolution and understand disease progression, response to treatment, and clinical outcomes

Reveal Tumor Heterogeneity and Cancer Mechanisms with New Single Cell Solutions from 10x Genomics

Single Cell Immune Profiling
Characterize complex tumor samples at single cell resolution
Single Cell Gene Expression Solution

Understanding what cell types and mutations are driving cancer depends on the ability to identify the cellular components of a tumor and dissect cell type differences. With our Chromium Single Cell Gene Expression Solution, you can characterize the gene expression profiles of hundreds to tens of thousands of tumor cells at single cell resolution:

  • Detect even more unique transcripts per cell with our latest sensitivity improvements
  • Identify novel and rare cell types or discover new cancer biomarkers
  • Determine targets for personalized therapy
  • Predict and measure therapy response
Single Cell Immune Profiling
Dissect genetic pathways that drive malignancy and tumor progression
Single Cell Gene Expression Solution with CRISPR Screening

Pooled CRISPR screening is a powerful method for identifying genes involved in complex biological mechanisms such as tumor cell proliferation, cancer cell survival, and drug/toxin resistance. In order to dissect the molecular pathways that underlie cancer progression and development, you need the ability to identify precise CRISPR perturbations—and associated molecular phenotypes—on a cell-by-cell basis. With our solution, you can:

  • Simultaneously identify CRISPR/Cas9 perturbations and their effects on gene expression from the same cancer cell using barcoded guide RNAs
  • Implement high-throughput CRISPR-based genetic screens in hundreds to tens of thousands of cells and detect complex cellular phenotypes
  • Dissect the genetic pathways driving malignancy and tumor progression
Single Cell Gene Expression
Combine cell surface tumor marker detection with single cell transcriptome measurements
Single Cell Gene Expression Solution with Cell Surface Protein

Oncogenesis leads to changes in the array of proteins and protein isoforms present on the cancer cell surface. These changes are not always reflected in the RNA expression profile of the cell. A multitude of cell surface marker proteins have been identified that distinguish cell types and cell states within a tumor. Leverage our Feature Barcoding technology to label cells with DNA-barcoded antibodies against cell surface proteins in your single cell sequencing experiments. With our solution, you can:

  • Integrate measurements of cell surface proteins and transcriptomes into a single readout
  • Further differentiate cancer cell populations by protein isoform expression measurements
  • Investigate cellular phenotypes in hundreds to tens of thousands of tumor cells
  • Identify novel treatment targets and biomarkers
Single Cell Gene Expression
Pair adaptive immune repertoire and cellular gene expression measurements for immunotherapy development
Single Cell Immune Profiling Solution

Harnessing the host immune response can lead to powerful anti-cancer therapies. Antigens within tumors can activate T cells, while at the same time, tumor cells begin to adapt to evade host immunity. Our Single Cell Immune Profiling Solution allows you to characterize tumor immune repertoires alongside gene expression in hundreds to tens of thousands of single cells. With this solution, you can:

  • Gain a detailed molecular understanding of the tumor environment and immune response
  • Sequence paired full-length lymphocyte receptors on a cell-by-cell basis for unbiased clonotype analysis
  • Accelerate the identification of anti-cancer immunotherapies
Single Cell Gene Expression
Simultaneously measure tumor cell types, antigens, and their paired TCR sequences
Single Cell Immune Profiling Solution with Antigen Specificity

Within tumors, major histocompatibility complex (MHC) cell surface proteins present antigens to T cells, launching the adaptive immune response. In research, MHC tetramers are a tool to identify the antigen binding specificity of infiltrating T cells. Our Feature Barcoding technology uses DNA barcodes to label MHC tetramers so you can measure tumor antigen specificity with single cell sequencing. With this solution, you can:

  • Obtain simultaneous readouts of transcriptomes, immune repertoires, paired T-cell receptor sequences, and target antigens on a cell-by-cell basis
  • Study dynamic interactions between lymphocytes and target cells within the tumor microenvironment
  • Map immune cell responses to specific pathogens to enhance CAR-T or TCR cell therapies
Single Cell Gene Expression
Integrate measurements of cell surface proteins, transcriptomes, and the immune repertoire into a single readout
Single Cell Immune Profiling Solution with Cell Surface Protein

A comprehensive phenotypic characterization of the various immune cells in a tumor can provide powerful insights into disease progression and potential treatments. Our Single Cell Immune Profiling Solution with Feature Barcoding technology for Cell Surface Protein integrates measurements of cell surface proteins, transcriptomes, and the immune repertoire into a single readout. With this solution, you can:

  • Distinguish protein isoforms for better characterization of tumor microenvironments and tumor-infiltrating lymphocytes
  • Differentiate between T- and B-cell clones mounting a successful immune response and those that have been evaded by the evolving tumor
  • Discover new biomarkers and therapy targets
Single Cell Immune Profiling
Unravel the epigenetic basis of cancer development and progression at single cell resolution
Single Cell ATAC Solution

Cancer is widely known to have a genetic basis, but more recently, chromatin and epigenetic aberrations have been implicated in its development and progression. Investigating epigenetic mechanisms can reveal critical insights into the control of signaling pathways involved in cancer. Our Single Cell ATAC Solution profiles the open chromatin landscape in hundreds to tens of thousands of individual nuclei. With this solution, you can:

  • Identify differences in epigenetic regulation among tumor cells
  • Uncover epigenetic mechanisms driving oncogenesis, tumorigenesis, and metastasis
  • Study the impact of epigenetic plasticity on clinical outcomes
Single Cell CNV
Unmask genetic heterogeneity and map clonal evolution in tumor populations
Single Cell CNV Solution

Somatic variation, drift, and selection rapidly create heterogeneity within a tumor. As a result, tumor subclones can show significant differences in copy number and overall ploidy. Characterizing cell-to-cell genetic diversity is important for understanding tumor progression and implementing personalized therapies. While bulk copy number profiling only provides an average ploidy measurement, our Single Cell CNV Solution detects copy number variation in hundreds to thousands of individual cancer cells. With this solution, you can:

  • Unmask the true genetic diversity of a tumor sample
  • Identify different tumor subclones and map clonal evolution
  • Correlate clonal genotypes with disease progression, response to treatment, and clinical outcomes
Get a Multidimensional View of Complex Cellular Systems with New Single Cell Solutions from 10x Genomics
Contact Me Request Pricing

Order your 10x system, today.