单细胞基因表达
通过单细胞转录组学揭示细胞多样性的错综复杂之处
Chromium单细胞基因表达解决方案
超越传统的基因表达分析
Chromium单细胞基因表达解决方案提供了一个全面且可扩展的解决方案，能够对数百个至数万个细胞进行细胞鉴定和基因表达分析。我们最新的升级让您能够从每个细胞中检测到更多的独特转录本，有了Feature Barcoding技术，您还能从每一个细胞中获得更完整的分子结果，从而鉴定CRISPR介导的细胞特异性扰动，或在同一细胞中同时测定基因表达和细胞表面蛋白表达，并在其他更多的细胞特征类型上更是有着几乎无限可能。
超越传统的基因表达分析，提升到在单细胞的基础上鉴定细胞群体、细胞类型、细胞状态等。从评估肿瘤异质性和干细胞组成，到剖析神经元群体，Chromium单细胞基因表达解决方案带来的技术进步以及一体化的软件工具，能够从单细胞中生成高复杂度的文库，从而实现在多种类型的样品中获得最大量的信息。
- 鉴定和描绘罕见的细胞类型
- 分析和了解细胞异质性以及它们对生物系统的贡献
- 利用单细胞RNA-seq开展细胞表型分析，鉴定新的靶点、生物标志物、细胞类型和状态，而无需预先选择靶点
- 评估同一细胞中的mRNA和细胞表面蛋白表达
- 对数万个细胞同时开展高通量、高分辨率的功能性遗传筛选
- 全面评估单独的CRISPR扰动后的基因表达表型
基因表达分析
揭开细胞之间的基因表达差异，鉴定异质性样品中的稀有细胞，从而更好地描绘细胞在发育和疾病中发挥的作用。我们最新的升级改进大大提高了灵敏度，让您能够从每个细胞中检测出更多独特的转录本。
Feature Barcoding技术：细胞表面蛋白
我们全新改进的工作流程采用Feature Barcoding技术，让您能更好地实现CITE-Seq/REAP-Seq等技术已发表的方法。可无缝检测同一细胞中的基因表达和细胞表面蛋白表达，以鉴定蛋白质异构体，检测转录本丰度低的蛋白质，并进一步增加表型特异性。
Feature Barcoding技术：CRISPR筛选
利用Feature Barcoding技术鉴定CRISPR介导的扰动以及对应的细胞转录本。这种全新改进的方法让您能够在数百至数万个单细胞中同时开展高通量且可扩展的功能性遗传筛选。
使用这些系统功能解锁单细胞分析
- 高效生成 100-80,000+ 个单细胞
- 出色的灵敏度，且细胞捕获率高达65%
- 简单的实验流程
- 对细胞大小要求更灵活，不设下限
- 双重率低，每1,000个细胞低于0.9%
- 可使用Next GEM技术
入门指南：
单细胞基因表达
无论您是想了解如何设计实验和优化参数，还是想知道如何确定计算/分析工具才能最好地分析您的单细胞基因表达数据，这本指南都一应俱全。立即下载入门指南，充分享受单细胞转录组学技术带来的丰富信息！
下载入门指南让每个细胞都有价值
观看工作原理
每次运行时分析数千个单细胞。了解10x技术如何开展数百万个并行反应，并以单细胞分辨率开展大规模基因表达分析。
Feature Barcoding技术
观看工作原理
了解我们融合了Feature Barcoding技术的解决方案将如何帮助您深入探索复杂的生物系统。观看实例，了解如何逐个细胞地获得更完整的结果，而未来更是有几乎无限可能。
Chromium Controller
小巧，时尚，高效。
小巧而时尚的Chromium Controller快速有效地结合了大量分区数量和大量不同的条形码库，能够在几分钟内高效地自动化生成大于10万个含有条形码的分区，从而实现高度并行的样品分液和添加分子标签。在Chromium Controller上，用户可以运行任何Chromium解决方案——从基因组到单细胞分析
了解Chromium ControllerChromium Connect
使用我们的自动化解决方案制备一致的单细胞基因表达文库
Chromium Connect将单细胞分区、条形码和文库构建整合在一个标准化、自动化的工作流程中，让您可以无人值守地实现从细胞到可以测序的文库制备。
下载产品说明
产品列表
在查看根据应用类型排列的产品列表时，首先选择您所需的单细胞基因表达解决方案版本。如果您希望将该解决方案与Feature Barcoding技术相结合，然后选择Feature Barcode的类型。
试剂和耗材
反应
产品代码
3′ CellPlex Kit Set A
48 rxns
1000261
3′ CellPlex Kit Set A
48 rxns
1000261
3′ Feature Barcode Kit
16 rxns
1000262
3′ Feature Barcode Kit
16 rxns
1000262
Chromium Next GEM Automated Single Cell 3′ Library and Gel Bead Kit v3.1
1000147
1000141
Chromium Next GEM Chip G Automated Single Cell Kit
1000146
1000136
Chromium Next GEM Chip G Single Cell Kit
1000127
1000120
Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v3.1
1000128
1000121
Chromium Next GEM Single Cell 3′ Kit v3.1
1000269
1000268
Chromium Next GEM Single Cell 3’ HT Kit v3.1
1000370
1000348
Chromium Next GEM Single Cell 3’ Library Construction Kit v3.1 (Optional)
16 rxns
1000157
Chromium Next GEM Single Cell 3’ LT Kit v3.1
4 rxns
1000325
Chromium Single Cell 3′ Feature Barcode Library Kit
16 rxns
1000079
Chromium Single Cell 3′ Library & Gel Bead Kit v2
120267
120237
Chromium Single Cell A Chip Kit
1000009
120236
Dual Index Kit NN Set A (for Sample Multiplexing)
96 rxns
1000243
Dual Index Kit NT Set A (for Feature Barcode Libraries)
96 rxns
1000242
Dual Index Kit NT Set A (for Feature Barcode Libraries)
96 rxns
1000242
Dual Index Kit TT Set A (for Gene Expression Libraries)
96 rxns
1000215
Dual Index Kit TT Set A (for Gene Expression Libraries)
96 rxns
1000215
Human Gene Signature Panel
1000258
1000245
Human Immunology Panel
1000259
1000246
Human Neuroscience Panel
1000277
1000278
Human Pan-Cancer Panel
1000260
1000247
Library Amplification Kit
16 rxns
1000249
Library Construction Kit
48 rxns
1000352
Library Construction Kit (Optional)
16 rxns
1000190
Library Construction Kit (Optional)
16 rxns
1000190
Single Index Kit T Set A
96 rxns
1000213
Target Hybridization Kit
16 rxns
1000248
Compatible Partner Products
仪器
保修期
产品代码
Chromium Connect
1000171
1000180
Chromium Controller & Next GEM Accessory Kit
1000202
1000204
Chromium Controller & Next GEM Accessory Kit
120223
120246
Chromium Controller & Next GEM Accessory Kit
1000202
1000204
Chromium iX & Accessory Kit
1000328
1000329
Chromium iX & Accessory Kit
1000328
1000329
Chromium X & Accessory Kit
1000331
1000332
Chromium X & Accessory Kit
1000331
1000332
Chromium X Upgrade Package
1000330
Chromium X Upgrade Package
1000330
软件
10x Genomics Cloud Analysis (US-only)
Cell Ranger Analysis Pipelines
Cell Ranger Analysis Pipelines
Custom Panel Designer
Loupe Browser
Loupe Browser
文档
Chromium Next GEM Automated Single Cell 3ʹ Reagent Kits User Guide (v3.1 Chemistry)
Coming soon
Chromium Next GEM Single Cell 3′ HT Reagen Kits v3.1 (Dual Index) User Guide
Coming soon
Chromium Next GEM Single Cell 3′ HT Reagen Kits v3.1 (Dual Index) with Feature Barcode technology for Cell Mulitplexing User Guide
Coming soon
Chromium Next GEM Single Cell 3′ HT Reagen Kits v3.1 (Dual Index) with Feature Barcode technology for Cell Surface Protein and Cell Multiplexing User Guide
Coming soon
Chromium Next GEM Single Cell 3′ HT Reagen Kits v3.1 (Dual Index) with Feature Barcode technology for Cell Surface Protein User Guide
Coming soon
Chromium Next GEM Single Cell 3′ HT Reagen Kits v3.1 (Dual Index) with Feature Barcode technology for CRISPR Screening and Cell Multiplexing User Guide
Coming soon
Chromium Next GEM Single Cell 3′ HT Reagen Kits v3.1 (Dual Index) with Feature Barcode technology for CRISPR Screening User Guide
Coming soon
Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 (Dual Index) with Feature Barcode technology for Cell Multiplexing
Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 (Dual Index) with Feature Barcode technology for Cell Multiplexing
Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 (Dual Index) with Feature Barcode technology for Cell Surface Protein and Cell Multiplexing
Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 (Dual Index) with Feature Barcode technology for CRISPR Screening and Cell Multiplexing
Chromium Next GEM Single Cell 3ʹ LT Reagent Kits v3.1 (Dual Index) User Guide
Chromium Next GEM Single Cell 3ʹ LT Reagent Kits v3.1 (Dual Index) with Feature Barcode technology for Cell Surface Protein User Guide
Chromium Single Cell 3′ Reagent Kits User Guide (v3.1 Chemistry)
Chromium Single Cell 3′ Reagent Kits User Guide (v3.1 Chemistry) with Feature Barcoding technology for Cell Surface Protein
Chromium Single Cell 3′ Reagent Kits User Guide (v3.1 Chemistry) with Feature Barcoding technology for CRISPR Screening
Chromium Single Cell 3′ Reagent Kits User Guide (v3.1 Chemistry – Dual Index)
Chromium Single Cell 3′ Reagent Kits User Guide (v3.1 Chemistry – Dual Index)
Chromium Single Cell 3′ Reagent Kits User Guide (v3.1 Chemistry – Dual Index) with Feature Barcode technology for Cell Surface Protein
Chromium Single Cell 3′ Reagent Kits User Guide (v3.1 Chemistry – Dual Index) with Feature Barcode technology for CRISPR Screening
Demonstrated Protocol: Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols
Demonstrated Protocol: Cell Multiplexing Oligo Labeling for Single Cell RNA Sequencing Protocols
Demonstrated Protocol: Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols
Demonstrated Protocol: Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols
Pre-designed Panel Gene Lists
Targeted Gene Expression Library Preparation User Guide – Single Cell
Technical Note: Chromium Next GEM Single Cell 3′ LT v3.1: Reagent, Workflow, & Data Overview
Technical Note: Chromium Next GEM Single Cell 3ʹ v3.1: Cell Multiplexing
Technical Note: Guide RNA Specifications Compatible with Feature Barcoding Technology for CRISPR Screening
User Guide : Chromium Single Cell 3′ Reagent Kits (v3 Chemistry) with CRISPR Screening Feature Barcoding Technology
User Guide: Chromium Single Cell 3′ Reagent Kits (v3 Chemistry)
User Guide: Chromium Single Cell 3′ Reagent Kits (v3 Chemistry) with Cell Surface Protein Feature Barcoding Technology
User Guide: Chromium Single Cell 3′ Reagents v2
单细胞基因表达软件
从10x软件支持中了解更多单细胞基因表达数据集
Chromium Demonstration (v2 Chemistry)
应用指南
海报墙
Spatially Resolved Molecular Investigation of Triple Negative Breast Cancer
- AMP 2020VirtualNovember 2020
High throughput immune profiling using integrated single cell multi-omics analyses
- Single Cell Biology Keystone SymposiaBreckenridge, COJanuary 2019