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Highly Multiplexed Transcriptome and Protein Immune Profiling in Single Cells with CITE-seq

May 23, 2018 @ 9:00 am - 10:30 pm PDT

| Free

High-throughput single-cell genomics has transformed our understanding of complex cell populations. Oligonucleotide-labeled antibodies allow integration of cellular protein and transcriptome measurements at a single-cell level, with the number of protein markers assayed simultaneously far surpassing what can be measured by cytometry-based approaches.

We present an overview of CITE-seq and Cell Hashing; methods compatible with 10x Genomics single cell platforms for enhanced cellular phenotyping and sample multiplexing, respectively. We demonstrate the advantages of using these methods for simultaneous measurement of protein and transcripts in single cells applied to T cell populations in the human immune system using a panel of ~80 antibodies.

Peter Smibert, Ph.D.
Manager, Technology Innovation
New York Genome Center

In this webinar, you’ll learn about:

  • An introduction to antibody-based methods for enhancing single cell RNA-seq
  • Advantages of sample multiplexing in scRNAseq experiments
  • Enhanced phenotyping through multi-omic single cell measurements
  • New capabilities of CITE-seq