Revolutionize your understanding
of neural cell diversity

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Reveal the Full Complexity of Neuronal Diversity
with Single Cell Analysis

Neural populations are dynamic, with diverse cell fates, states, and functions. Unraveling these multiple dimensions requires the ability to identify gene expression profiles, trace cellular lineage, and profile cell subtypes. Biased cellular analysis and pooled samples, as well as cumbersome dissection and staining techniques, have prevented neuroscience researchers from uncovering the intricacy of neural events.

With single cell sequencing technologies from 10x Genomics, you can get novel insight into the molecular mechanisms underlying both normal cellular function and disease states.

  • Comprehensively characterize intracellular heterogeneity, neural cell types and states, neuronal activation, and dynamic cellular transitions in hundreds to tens of thousands of brain cells
  • Go beyond the need for pre-selected targets and identify rare or novel neural populations and cell subtypes with unbiased cell-by-cell transcript and genomic heterogeneity measurements
  • Gain insight into cellular function and the molecular mechanisms underlying neurological disease by profiling gene expression and cell surface proteins, mapping the chromatin landscape, and revealing the full spectrum of cellular genomes in single neurons

Get a Multidimensional View of Complex Neuronal Systems with New Single Cell Solutions from 10x Genomics

Single Cell Immune Profiling
Characterize heterogeneous brain cell populations
Single Cell Gene Expression

During development, neural progenitor cells undergo dynamic cellular and molecular changes to generate a diverse population of neurons, astrocytes, and oligodendrocytes. Dynamic changes also occur in disease and in differentiated cells underlying normal neurological processes. Defining the complexity of subpopulations of cells will help provide insight into cellular dysregulation in neurological disorders and increase our understanding of developmental processes. With 10x Genomics’ Single Cell Gene Expression Solution, you can:

  • Analyze individual cellular transcriptomes to identify activated neurons and rare neural cell types and states without the limits of target pre-selection
  • Profile populations and inter-relationships of subtypes of neurons, astrocytes, and oligodendrocytes
  • Gain a detailed understanding of cellular function and the molecular mechanisms underlying neurological development and disease
Single Cell Immune Profiling
Measure surface markers together with gene expression on a cell-by-cell basis
Single Cell Gene Expression Solution with Cell Surface Protein

Cell surface marker expression has been used to identify and isolate hundreds of neural cell types by FACS of embryonic and adult tissue from multiple species. Additionally, complex signaling via cell surface proteins controls the rapid communication of information within the brain. Cell surface protein identification is critical for studying both rapid and neuromodulatory signaling in the brain, identifying disease biomarkers, and further differentiating neural cell types. By combining our Single Cell Gene Expression Solution with Feature Barcoding technology to measure surface protein expression, you can:

  • Integrate measurements of cell surface proteins and transcriptomes into a single readout
  • Map neuronal and non-neuronal cell types, signaling pathways, and networks without the need for flow sorting or pre-enrichment
  • Further differentiate brain cell populations by protein isoform expression measurements
  • Identify novel cell types based on combined protein and transcript information
Single Cell Immune Profiling
Track epigenetic changes during neural development
Single Cell ATAC Solution

The control of gene expression via epigenetic mechanisms integrates both intrinsically programmed and environmental signals in neurons. Analyzing the epigenome at the single cell level is critical in understanding how neuronal populations develop and regulate their expression profiles. Extend your analysis of gene regulatory networks with the Single Cell ATAC Solution:

  • Profile the chromatin landscape cell by cell and determine the role of nucleosomes, chromatin compaction, and DNA-binding proteins in regulating gene expression in neural populations
  • Uncover cellular epigenetic variability
  • Dissect the role of epigenetics in neural plasticity
Single Cell CNV
Unmask genetic heterogeneity and map neuronal mosaicism
Single Cell CNV Solution

Cytogenomic mutations and chromosomal abnormality are implicated in the neuropathology of several brain diseases. Brain tissue is extremely heterogeneous, making it difficult to detect and validate these changes. When off-target cells dilute the abnormal genome population, more sensitivity is required. With our Single Cell CNV Solution, you can:

  • Detect copy number variation in hundreds to tens of thousands of individual cells to unmask genetic heterogeneity and reveal distinct genetic populations
  • Identify neuronal mosaicism and how this may be affecting development and disease
  • Gain a deeper understanding of cell lineage in neural development and disease pathogenesis
Get a Multidimensional View of Complex Cellular Systems with New Single Cell Solutions from 10x Genomics
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