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Fixed RNA Profiling

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What is Fixed RNA Profiling?

Fixed RNA Profiling enables single cell gene expression on precious samples that were previously inaccessible due to logistical challenges in sample handling. Fixation at the point of sample collection preserves fragile biology and greatly streamlines workflows to provide critical insights on more samples.

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Documentation

We provide resources from protocols, user guides, and free-to-use analysis tools.

Experimental Design & Planning

Plan ahead by referring to the list of equipment, kits, and reagents needed for Fixed RNA Profiling.

Sample Prep

Prepare optimal single cell/nuclei suspension then fix cells for immediate processing or storage by following our Demonstrated Protocols.

Probe Sets

Find a list of probes targeting protein-coding genes in human transcriptome for the Fixed RNA Profiling assay.

Library Construction

Construct a sequencing-ready single cell library by following our step-by-step User Guide / Protocol.

Instruments

Refer to instrument-specific documents for seamless instrument operation.

Sequencing

Sequence your genome and exome library as per our provided recommendations.

Software Analysis

Analyze your genome and exome data using our pipelines and visualization tools.

Additional Applications

Cell Surface Protein
Reveal gene expression and cell surface protein from the same fixed cell.
Datasets

Access free, publicly available data from a number of different sample types generated with Chromium single cell solutions.

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Datasets

Videos

More videos for Fixed RNA Profiling are coming soon.

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Q&A

Find answers to common technical questions, from sample prep through data analysis, about the Fixed RNA Profiling workflow.

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Q&A