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Provides step-by-step guidance on how to perform tissue fixation and dissociation for Fixed RNA Profiling.

Tissue Fixation & Dissociation for Chromium Fixed RNA Profiling

Prepare optimal single cell/nuclei suspension then fix cells for immediate processing or storage by following our Demonstrated Protocols.

Sample Prep

Fixed RNA Profiling enables single cell gene expression on precious samples that were previously inaccessible due to logistical challenges in sample handling. Fixation at the point of sample collection preserves fragile biology and greatly streamlines workflows to provide critical insights on more samples.

Plan ahead by referring to the list of equipment, kits, and reagents needed for Fixed RNA Profiling.

Experimental Design

Experimental Design & Planning

Find a list of probes targeting protein-coding genes in human transcriptome for the Fixed RNA Profiling assay.

Probe Sets

Construct a sequencing-ready single cell library by following our step-by-step User Guide / Protocol.

Library Construction

Refer to instrument-specific documents for seamless instrument operation.

Instruments

Sequence your genome and exome library as per our provided recommendations.

Sequencing

Analyze your genome and exome data using our pipelines and visualization tools.

Software Analysis

Fixed RNA Profiling

Overview

Documentation

Additional Applications

Datasets

Videos

What is Fixed RNA Profiling?

Reveal gene expression and cell surface protein from the same fixed cell.

Cell Surface Protein

We provide resources from protocols, user guides, and free-to-use analysis tools.

Access free, publicly available data from a number of different sample types generated with Chromium single cell solutions.

More videos for Fixed RNA Profiling are coming soon. 

Find answers to common technical questions, from sample prep through data analysis, about the Fixed RNA Profiling workflow.

Tissue Fixation & Dissociation for Chromium Fixed RNA Profiling

10x Genomics Sample Preparation Products, CG000553

CG000553_DemonstratedProtocol_TissueFixationDissociation_RevA.pdf