We recently identified a bug in Chromium Connect software releases 20211214 and 20220114 that result in incorrect or incomplete instructions for loading and setup when starting runs for two to six samples. This guidance document is available to help you proceed with running two to six samples with the affected workflows. Please see below for all available PDFs based off of affected assay and sample count
Chromium Next GEM Automated Assay/Kits requiring a deviation in workflow:
| 10x Genomics Chromium Next GEM Automated Assay/Kits | Number of Samples | UI screens PDF |
|---|---|---|
| Automated Single Cell 3’ Reagent Kits v3.1 (Single index) | 2-3 Samples | Automated Single Cell 3’ Reagent Kits v3.1 (Single index with 2 to 3 samples) |
| Automated Single Cell 3’ Reagent Kits v3.1 (Single index) | 4-6 Samples | Automated Single Cell 3’ Reagent Kits v3.1 (Single index with 4 to 6 samples) |
| Automated Single Cell 3’ Reagent Kits v3.1 (dual index) | 2-3 Samples | Automated Single Cell 3’ Reagent Kits v3.1 (Dual Index with 2 to 3 samples) |
| Automated Single Cell 3’ Reagent Kits v3.1 (dual index) | 4-6 Samples | Automated Single Cell 3’ Reagent Kits v3.1 (Dual Index with 4 to 6 samples) |
| Automated Single Cell 5’ Reagent Kits v2 | 2-3 Samples | Automated Single Cell 5’ Reagent Kits v2 (2 to 3 samples) |
| Automated Single Cell 5’ Reagent Kits v2 | 4-6 Samples | Automated Single Cell 5’ Reagent Kits v2 (4 to 6 samples) |
| Automated Single Cell V(D)J Amplification Kit | 3-5 Samples | Automated Single Cell V(D)J Amplification Kit (3 to 5 samples) |