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Chromium Next GEM Single Cell 3' LT v3.1: Reagents, Workflow, & Data Overview

  • CG000406_TechNote_ChromiumNextGEMSingle Cell 3' LT Kit Workflow and data overview_RevA.pdf

    Technical Note, CG000406

    CG000406_TechNote_ChromiumNextGEMSingle Cell 3' LT Kit Workflow and data overview_RevA.pdf

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Chromium Next GEM Single Cell 3’ LT v3.1 is a low throughput, cost-effective solution for smaller-scale and pilot studies, which enables profiling whole transcriptome at the single cell level for one hundred to a thousand cells per sample. In combination with Feature Barcode technology, the assay also enables simultaneous cell surface protein detection in single cells. This Technical Note highlights sample preparation, reagents, and workflow specifics for Single Cell 3’ LT v3.1, along with information about cell dilution, cell multiplet rate, signal-to-noise data profile, and cluster defining for low cell loads. A comparison of representative data derived from the low throughput Single Cell 3’ LT v3.1 assay versus the standard Single Cell 3’ v3.1 assay is also presented.

Document Type
Technical Note

Last Modified
March 31, 2021