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Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing

  • 20190802_CG00014_FreshFrozenHumanMouseCellLineMix_RevEtoRevF.pdf

    Demonstrated Protocol, CG00014

    20190802_CG00014_FreshFrozenHumanMouseCellLineMix_RevEtoRevF.pdf

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  • CG00014_Demonstrated Protocol FreshFrozenHumanMouseCellLineMix_RevG.pdf

    Demonstrated Protocol, CG00014

    CG00014_Demonstrated Protocol FreshFrozenHumanMouseCellLineMix_RevG.pdf

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The ability of the 10x Genomics® Single Cell Solutions to partition single cells in a heterogeneous population can be verified by profiling a mixture of cells from two different species. Ideally, all sequence reads from a single Gel Bead-in-EMulsion (GEM) will be unambiguously mapped to the transcriptome of only one of the two species.

This demonstrated protocol is optimized for counting cells in the range of 700-1200 cells/µl. If using the Single Cell 3' LT v3.1 (low throughput) application, ensure cells are counted as indicated in this protocol and then diluted to the LT specific optimal loading concentration of 100-600 cells/µl using the Cell Dilution Overview in the LT User Guide.
Document Type
Demonstrated Protocol

Last Modified
June 17, 2022