| Library Combination | CR 8.0 | CR 7.2 and lower |
|---|---|---|
| 3’ Gene Expression v4 | - | |
| 3’ Gene Expression v4 + Antibody Capture | - |
Chromium GEM-X Single Cell Gene Expression v4 chemistry does not support the following library types:
- 3' Gene Expression v4 + CellPlex (not supported)
- 3' Gene Expression v4 + CRISPR Guide Capture (not supported)
Accordingly, Cell Ranger v8.0 cannot process these libraries.
| Library Combination | CR 8.0 | CR 7.2 | CR 7.1 | CR 7.0 | CR 6.1 | CR 6.0 | CR 5.0 | CR 4.0 | CR 3.1 | CR 3.0 | CR 2.2 |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 3’ Gene Expression v2 | |||||||||||
| 3’ Gene Expression v3 | - | ||||||||||
| 3’ Gene Expression v3 + Cell Surface Protein | - | ||||||||||
| 3’ Gene Expression v3 + CRISPR Screening Expression | - | ||||||||||
| 3’ Cell Surface Protein only | - | - | |||||||||
| 3’ Targeted Gene Expression | - | - | - | - | - | ||||||
| 3’ Cell Multiplexing | - | - | - | - | - | ||||||
| 3’ LT (Low Throughput) | - | - | - | - | - | - | - | ||||
| 3’ HT (High Throughput) | - | - | - | - | - | - |
| Library Combination | CR 8.0 | CR 7.2 and lower |
|---|---|---|
| 5’ Gene Expression v3 | - | |
| 5’ Gene Expression v3 + V(D)J | - | |
| 5’ Gene Expression v3 + Cell Surface Protein | - | |
| 5’ Gene Expression v3 + Cell Surface Protein + V(D)J | - | |
| 5’ Gene Expression v3 + Cell Surface Protein + CRISPR | - | |
| 5’ Gene Expression v3 + CRISPR | - | |
| 5’ Gene Expression v3 + V(D)J + CRISPR | - | |
| 5’ Gene Expression v3 + CSP + V(D)J + CRISPR | - |
Chromium GEM-X Single Cell Immune Profiling v3 chemistry does not support the following library types:
- 5' Gene Expression v3 + V(D)J + Antigen Capture (not supported)
However, Cell Ranger v8.0 can process 5' Gene Expression + V(D)J libraries + Antigen Capture generated using Next GEM v2 chemistry (see below).
| Library Combination | CR 8.0 | CR 7.2 | CR 7.1 | CR 7.0 | CR 6.1 | CR 6.0 | CR 5.0 | CR 4.0 | CR 3.1 | CR 3.0 | CR 2.2 |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 5’ Gene Expression | |||||||||||
| 5’ V(D)J | |||||||||||
| 5’ Gene Expression + V(D)J | |||||||||||
| 5’ Gene Expression + Cell Surface Protein | - | ||||||||||
| 5’ Gene Expression + Cell Surface Protein + V(D)J | - | ||||||||||
| V(D) J + Cell Surface Protein | - | ||||||||||
| 5’ Cell Surface Protein only | - | - | |||||||||
| 5’ Targeted Gene Expression | - | - | - | - | - | ||||||
| 5’ HT (High Throughput) | - | - | - | - | - | - | - | ||||
| 5’ Gene Expression + CRISPR | - | - | - | - | - | - | - | ||||
| 5’ Gene Expression + CRISPR + Cell Surface Protein | - | - | - | - | - | - | - | ||||
| 5’ Gene Expression + CRISPR + V(D)J | - | - | - | - | - | - | - | ||||
| 5’ Gene Expression + Antigen Capture + V(D)J ± Cell Surface Protein | - | - | - | - | - | - | - | - |
| Library Combination | CR 8.0 | CR 7.2 | CR 7.1 | CR 7.0 |
|---|---|---|---|---|
| Gene expression (singleplex or multiplex) | ||||
| Gene expression + Antibody Capture (singleplex) | ||||
| Single cell FFPE Gene Expression (singleplex or multiplex) | - | |||
| Gene expression + Antibody Capture (multiplex) | - | - | ||
| Gene expression + CRISPR (singleplex or multiplex) | - | - | - | |
| Gene expression + CRISPR + Antibody (singleplex or multiplex) | - | - | - |