Human hematopoietic/stem progenitor (HSPC) CD34+ cells are the core components of ex vivo gene therapy (GT) for the treatment of rare monogenic diseases. Assessment of CD34+ cells for their long-term hematopoietic stem cell (HSC) content before and after ex vivo transduction, as well as in vivo after transplantation, is a critical parameter to establish the efficacy of GT. To overcome the limitations of standard immunophenotyping, we leveraged the high resolution offered by single cell (sc)-RNA-sequencing to capture the heterogeneity of…

In this webinar, we will tell two stories of how we are using our Enhanced Single Cell Analysis with Protein Expression (ESCAPE™) RNA sequencing assay and MapCell AI™ with the 10x Genomics single cell analysis platform to tackle a diversity of problems in both cancer and infectious disease.

This webinar will discuss a study that combined single-cell gene expression and spatial gene expression to understand the evolution of sepsis in the kidney at the cellular and molecular level. Understanding the pathophysiology of acute kidney injury is crucial for the comprehensive treatment of sepsis and its complications, but the exact cellular and molecular contributors to this multifaceted response remain unknown. Furthermore, the kidney is a highly complex organ, in which epithelial, endothelial, immune, and stromal cells are at constant…

Without the limitations of a bulk CRISPR screen, what are the possibilities? With a high-resolution view of the transcriptome effects of CRISPR perturbations and increased speed to target, single cell CRISPR screening enables truly scalable functional genomics. Join the 10x Genomics Innovation Lab panel discussion and explore how recent advances in single cell methodology provide deeper insights into cellular response, pathways, and systems as compared to output from bulk screening.

In this GEN webinar, Dr. Katerina Akassoglou of the Gladstone Institutes will address the gaps in our understanding and treatment of neuroinflammation. Dr. Akassoglou will also describe how single-cell gene expression paired with small-molecule screening and pathway analysis provides an effective functional transcriptomic and drug discovery pipeline. Additionally, she will tell us how she and her team were able to utilize the Chromium Single Cell Gene Expression system from 10x Genomics to characterize the functional transcriptomic landscape of oxidative stress-inducing…