What is CytAssist Spatial Gene Expression for Fixed Frozen (FxF)?
Whole transcriptome analysis of mouse fixed frozen OCT embedded tissue sections utilizing probe-based chemistry.
This assay utilizes the following 10x products:
- Visium CytAssist Spatial Gene Expression for FFPE Reagent Kits
- Visium CytAssist Instrument and Accessory Kit, to learn more click here
This assay has been validated with the following:
- Paraformaldehyde (PFA) fixed, sucrose cryopreserved, OCT embedded tissue blocks
- 10-20 µm section thickness
- H&E Staining
- Tissue types (Tested Tissue List)
10x Visium Kit Compatibility Table
Analyte Capture Method | 10x Solution | FFPE Tissue | Fresh Frozen Tissue | Fixed Frozen Tissue |
---|---|---|---|---|
CytAssist Enabled, Probe-based chemistry | Visium CytAssist Spatial Gene Expression | |||
Direct Placement, Probe-based chemistry | Visium Spatial Gene Expression for FFPE | - | - | |
Direct Placement, Poly-A based chemistry | Visium Spatial Gene Expression for Fresh Frozen | - | - |