Workflow Documentation
Documentation for Fresh Frozen and Fixed Frozen Applications.
Plan ahead by referring to the list of equipment, kits, and reagents needed for the Visium CytAssist Spatial Gene Expression protocols.
Best practices for handling FFPE tissue samples and slides before and after sectioning. Guidance for removing hardset coverslips from archived tissue slides.
Deparaffinize, stain, image, and decrosslink FFPE tissue sections.
For imaging hardware, general image acquisition and analysis guidelines, and example images.
Find a list of probes targeting protein-coding genes in human or mouse transcriptome for the Visium CytAssist Spatial Gene Expression assay.
Construct a sequencing-ready Visium spatial library from your FFPE tissue sections.
Refer to instrument-specific documents for seamless instrument operation.
Sequence your Visium spatial library as per our provided recommendations.
Analyze your Visium spatial data using our pipelines and visualization tools.