10k 1:1 Mixture of Human HEK293T and Mouse NIH3T3 cells, 3' v3.1, Chromium Controller

Single Cell Gene Expression dataset analyzed using Cell Ranger 6.1.0

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Dataset overview

Output and supplemental files

Input files

Learn about Chromium analysis

1:1 mixture of fresh frozen human HEK293T and mouse NIH3T3 cells obtained by 10x Genomics from ATCC were used in a classic experiment to demonstrate single cell behavior.

Libraries were generated from ~16,000 cells (10,578 cells recovered) as described in the Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry Dual Index) (CG000315 Rev C) using the Chromium Controller and sequenced on an Illumina NovaSeq 6000 to a read depth of approximately 50,000 mean reads per cell.

Paired-end, dual indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI) i5 index: 10 cycles (sample index) i7 index: 10 cycles (sample index) Read 2: 90 cycles (transcript).

Run with --expect-cells=10000.

This dataset is licensed under the Creative Commons Attribution license.