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10k Human PBMCs with TotalSeq™-B Human TBNK Antibody Cocktail, 3’ v3.1

Universal 3' dataset analyzed using Cell Ranger 6.0.0

Number of samples on this datasets page1

Peripheral blood mononuclear cells (PBMCs) from a healthy donor were obtained by 10x Genomics from AllCells and were labelled with a panel of a panel of nine TotalSeq™-B antibodies (BioLegend).

Libraries were generated following the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index) with Feature Barcode technology for Cell Surface Protein and Cell Multiplexing User Guide (CG000390) and sequenced on Illumina NovaSeq 6000.

Sequencing Depth: 49,172 mean reads per cell

Paired-end, dual indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI) i5 index: 10 cycles (sample index) i7 index: 10 cycles (sample index) Read 2: 90 cycles (transcript)

Analysis Parameters Used: Run with --expect-cells=10000

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.

Product

Universal 3'

Additional Applications

Cell Surface Protein

Software

Cell Ranger

Subpipeline

cellranger count

10x Instrument(s)

Chromium Controller

Species

Human

Anatomical Entity

Blood

Disease State

healthy

Cells or Nuclei

Cells

Selected Cell Types

PBMCs

Biomaterial Type

Cell Suspension

Cell/Nuclei Count

9,273

Donor Count

1

Date Published

2021-03-31