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10k Human DTC Melanoma, Chromium NextGEM Single Cell 3'

Universal 3' dataset analyzed using Cell Ranger 8.0.0

Number of samples on this datasets page1

Dissociated tumor cells (DTCs) from melanoma tissue (female, age 50-60) were obtained by 10x Genomics from Discovery Life Sciences.

Gene Expression libraries were generated as described in the Chromium Single Cell 3' Reagent Kits User Guide (v3.1 - Dual Index) (CG000315) and sequenced on an Illumina NovaSeq 6000 with approximately 55,000 read pairs per cell.

Paired-end, dual indexing:

  • 28 cycles Read 1
  • 10 cycles i7
  • 10 cycles i5
  • 90 cycles Read 2

The cellranger multi pipeline in Cell Ranger v8.0.0 was used to analyze libraries.

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.

Product

Universal 3'

Software

Cell Ranger

Subpipeline

cellranger multi

10x Instrument(s)

Chromium X Series

Species

Human

Anatomical Entity

Blood

Preservation Method

Fresh Frozen

Cells or Nuclei

Cells

Selected Cell Types

dissociated tumor cells

Biomaterial Type

Cell Suspension

Cell/Nuclei Count

15,321

Donor Count

1

Date Published

2024-03-13