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5k 1:1 mixture of fresh frozen human (HEK293T) and mouse (NIH3T3) cells (Next GEM)

Universal 3' dataset analyzed using Cell Ranger 3.0.2

Number of samples on this datasets page1

1:1 mixture of fresh frozen human (HEK293T) and mouse (NIH3T3) cells.

This is a classic human-mouse mixture experiment to demonstrate single cell behavior (the same cells were used to generate 1k_hgmm_v3, 1k_hgmm_v3_nextgem, 10k_hgmm_v3_nextgem).

Libraries were prepared following the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 User Guide (CG000204 RevA).

  • 6,163 cells detected
  • Sequenced on Illumina NovaSeq with approximately 72,279 reads per cell
  • 28bp read1 (16bp Chromium barcode and 12bp UMI), 91bp read2 (transcript), and 8bp I7 sample barcode
  • run with --expect-cells=5000

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.

Product

Universal 3'

Software

Cell Ranger

Subpipeline

cellranger count

10x Instrument(s)

Chromium Controller

Species

Human, Mouse

Anatomical Entity

Embryonic Kidney, Embryo

Preservation Method

Fresh Frozen

Cells or Nuclei

Cells

Selected Cell Types

fibroblast

Biomaterial Type

Cell Line

Cell/Nuclei Count

6,163

Donor Count

2

Date Published

2019-05-29