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750 Sorted Cells from Human Invasive Ductal Carcinoma, 3’ LT v3.1

Universal 3' dataset analyzed using Cell Ranger 6.0.0

Number of samples on this datasets page1

Invasive Ductal Carcinoma cells from a female donor aged 65 were obtained by 10x Genomics from Discovery Life Sciences.

Libraries were prepared following the Chromium Next GEM Single Cell 3' LT Reagent Kits v3.1 (Dual Index) User Guide (CG000399) and sequenced on Illumina NovaSeq 6000.

Sequencing Depth: 62,379 mean reads per cell

Paired-end, dual indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI) i5 index: 10 cycles (sample index) i7 index: 10 cycles (sample index) Read 2: 90 cycles (transcript)

Analysis Parameters Used: Run with --expect-cells=750

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.

Product

Universal 3'

Additional Applications

Low Throughput

Software

Cell Ranger

Subpipeline

cellranger count

10x Instrument(s)

Chromium Controller

Species

Human

Anatomical Entity

Breast

Disease State

Invasive Ductal Carcinoma, breast cancer

Cells or Nuclei

Cells

Selected Cell Types

Sorted

Biomaterial Type

Specimen from Organism

Cell/Nuclei Count

687

Donor Count

1

Date Published

2021-03-31