Hodgkin's Lymphoma, Dissociated Tumor: Targeted, Gene Signature Panel

Single Cell Gene Expression dataset analyzed using Cell Ranger 4.0.0

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Dataset overview

Output and supplemental files

Input files

Learn about Chromium analysis

Human dissociated lymph node tumor cells of a 19-year-old male Hodgkin’s Lymphoma patient were obtained by 10x Genomics from Discovery Life Sciences.

Whole transcriptome libraries were generated with Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (Dual Index) User Guide (CG000315) and sequenced on an Illumina NovaSeq 6000. The targeted libraries were generated using the Targeted Gene Expression Reagent Kits User Guide (CG000293) and Human Gene Signature Panel reagent (PN-1000245) and sequenced on an Illumina NovaSeq 6000.

Targeted Gene Expression Library

Sequencing Depth: 10,149 mean reads per cell
Paired-end (28 x 90) dual indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI) i7 index: 10 cycles i5 index: 10 cycles Read 2: 90 cycles (transcript)

Key metrics were:

Number of cells called: 3,091
Reads Mapped Confidently to Targeted Transcriptome: 87.7%
Total targeted genes detected: 1125

This dataset is licensed under the Creative Commons Attribution license.