Human Glioblastoma Multiforme: 5’v1 Whole Transcriptome Analysis
Single Cell Immune Profiling dataset analyzed using Cell Ranger 4.0.0
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Dataset overview
Output and supplemental files
Input files
Learn about Chromium analysis
Human Glioblastoma Multiforme cells from a male donor aged 57 were obtained by 10x Genomics from Discovery Life Sciences. Whole transcriptome/gene expression libraries were generated as described in the Chromium Single Cell 5' Reagent Kits v1 User Guide (CG000086). This whole transcriptome library was used as the parent library for downstream target enrichment (see additional datasets).
Sequencing data was processed using Cell Ranger, with websummary and output files available below.
- Sequencing instrument: Illumina NovaSeq 6000
- Sequencing depth: 81,573 mean reads per cell
- Sequencing configuration: Paired-end (28 X 91), single-indexed. Read 1: 28 cycles (16 bp barcode, 10 bp UMI); i7 index: 8 cycles; i5 index: 0 cycles; Read 2: 91 cycles (transcript). Note: Read 1 requirement is 26 bp; 28 bp Read 1 is trimmed by Cell Ranger pipeline.
- Cells detected: 4,103
- Median genes per cell: 2,763
- Median UMIs per cell: 9,156
This dataset is licensed under the Creative Commons Attribution license.