Snap-frozen normal mouse kidney tissue was obtained by 10x Genomics from AcePix Biosciences. Nuclei were isolated following the Nuclei Isolation from Complex Tissues for Single Cell Multiome ATAC + Gene Expression Sequencing Demonstrated Protocol (CG000375, Rev D). Paired ATAC and Gene Expression libraries were generated as described in the Chromium GEM-X Epi Multiome User Guide (CG001689, Rev B) and sequenced on an Illumina NovaSeq 6000 (ATAC) and Illumina NovaSeqX+ (GEX) with approximately 40,000 ATAC read pairs per cell and 60,000 GEX read pairs per cell.
Gene Expression library, paired-end, dual indexed:
- 28 cycles Read 1
- 10 cycles i7
- 10 cycles i5
- 90 cycles Read 2
ATAC library, paired-end, single index:
- 50 cycles Read 1
- 8 cycles i7
- 24 cycles i5
- 49 cycles Read 2