PBMCs of a healthy donor - TCR enrichment from amplified cDNA

Peripheral blood mononuclear cells (PBMCs) of a healthy donor, purchased from AllCells (Catalog #: PB001), 87% viable by Trypan blue stain. PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).

The same cDNA was used to generate gene expression and cell surface protein (vdj_v1_hs_pbmc2_5gex_protein) and B cell Ig (vdj_v1_hs_pbmc2_b) specific libraries.

Libraries were prepared following the Single Cell V(D)J Reagent Kits User Guide (CG000186 RevA).

• ~17,000 total cells loaded
• 3,719 T cells expected based on CD3D/CD3E expressing fraction in 5’GEX data
• 3,847 T cells expected based on CD3+ fraction in antibody data (CD3+ antibody cells identified by gating on UMI counts relative to IgG1 isotype control)
• 3,308 cells detected with either single TRA or TRB or both
• 75% cells with productive V-J spanning (TRA,TRB) pair
• Sequenced on an Illumina HiSeq4000 with ~10,000 read pairs per cell
• 150bp read1 (including 16bp Chromium barcode, 10bp UMI, 13bp switch oligo and transcript), 150bp read2 (transcript), and 8bp I7 sample barcode read configuration

This dataset is licensed under the Creative Commons Attribution license.