MultiPro®
The MultiPro Human Discovery Panel contains 347 DNA-barcoded antibodies used to profile up to 326 protein targets. The Panel contains 5 isotype control antibodies. This product has been designed and validated across diverse sample types and conditions with our Flex single cell assays with Feature Barcode technology.Human Discovery Panel
Validation data gallery
Lewis x antigen involved in cell-cell adhesion, mediates phagocytosis and chemotaxis. UMAP shows resting bone marrow mononuclear cells
E-cadherin, mediates cell-cell adhesion in epithelial tissues. UMAP shows resting bone marrow mononuclear cells
Stem cell factor receptor, plays essential roles in hematopoiesis, spermatogenesis, and melanogenesis. UMAP shows resting bone marrow mononuclear cells
Protein kinase C and casein kinase substrate in neurons protein 1, involved in endocytosis and actin dynamics. UMAP shows human PBMCs stimulated with anti-CD3/CD28 for 4 hr
H2A histone family member X, involved in DNA repair and chromatin structure. UMAP shows human PBMCs stimulated with anti-CD3/CD28 for 4 hr
Transcription factor critical for hematopoietic differentiation and lymphatic system development. UMAP shows resting bone marrow mononuclear cells and in human PBMCs stimulated with LPS for 4 hr
Drebrin 1, involved in dendritic spine morphogenesis and synaptic plasticity. UMAP shows human PBMCs stimulated with LPS for 4 hr
Transcription factor critical for hematopoietic differentiation and lymphatic system development. UMAP shows resting bone marrow mononuclear cells and in human PBMCs stimulated with LPS for 4 hr
Stem cell factor receptor, plays essential roles in hematopoiesis, spermatogenesis, and melanogenesis. UMAP shows resting bone marrow mononuclear cells
N-cadherin, involved in cell-cell adhesion and signal transduction, crucial in the nervous system and heart. UMAP shows resting PBMCs.
Phosphorylated MAP kinase kinase 1 at Serine 298, part of the MAPK/ERK pathway, involved in cell growth signaling. UMAP shows resting PBMCs.
Transforming growth factor beta 1, regulates cell growth, proliferation, differentiation, and apoptosis. UMAP shows resting PBMCs.
Product information
Tested reactivity
Human
Host / isotype
Rabbit polyclonal IgG
Mouse IgG2a
IgG2b
Mouse IgG2b
Mouse IgG1
IgMk
Mouse IgG3
Mouse IgG2ak
Class
Monoclonal
Recombinant
Polyclonal
Type
DNA-barcoded antibody cocktail
Storage buffer
Lyophilized antibody cocktail
Storage conditions
Store at 2–8°C.
Targets
MultiPro Discovery Antibody Manifest
*This file contains macros that are used to make searching the list of antibodies easier. Make sure to enable macros when prompted. You may also need to “unblock” the file in the file’s properties box.
Documentation
GEM-X protocols
Next GEM protocols
SDS
Feature References
*The use of MultiPro Enhanced Blocking Reagent is highly recommended for improved signal-to-noise ratio. See protocol for more details.
Sequencing parameters
If using Cell Ranger v8.0+
Read 1: 28 cycles
Index 1: 10 cycles
Index 2: 10 cycles
Read 2: 90 cycles
If using Cell Ranger v7.2
Read 1: 48 cycles
Index 1: 10 cycles
Index 2: 10 cycles
Read 2: 50 cycles