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1k PBMCs from a Healthy Donor - Gene Expression with a Panel of TotalSeq™-B Antibodies

Universal 3' dataset analyzed using Cell Ranger 3.0.0

Number of samples on this datasets page1

Peripheral blood mononuclear cells (PBMCs) from a healthy donor stained with a panel of TotalSeq™-B Antibodies. PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).

  • 713 cells detected
  • Sequenced on Illumina HiSeq4000 with approximately 63,000 reads per cell
  • 28bp read1 (16bp Chromium barcode and 12bp UMI), 91bp read2 (transcript), and 8bp I7 sample barcode
  • run with --expect-cells=1000
  • This dataset was generated using a pre-release set of TotalSeq-B antibodies. The barcode sequences have since changed. Please refer to https://www.biolegend.com/totalseq for the latest conjugated barcode information.

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.

Product

Universal 3'

Additional Applications

Cell Surface Protein

Software

Cell Ranger

Subpipeline

cellranger count

10x Instrument(s)

Chromium Controller

Species

Human

Anatomical Entity

Blood

Disease State

healthy

Cells or Nuclei

Cells

Selected Cell Types

PBMCs

Biomaterial Type

Cell Suspension

Cell/Nuclei Count

713

Donor Count

1

Date Published

2018-11-19