Technical Note - Base Composition of Sequencing Reads of Chromium Single Cell V(D)J Libraries

This Technical Note describes base percentage profiles and Phred quality scores (shown as %Q30 in Illumina SAV software) that are characteristic of Chromium Single Cell V(D)J libraries for the recommended sequencing run configuration. Chromium Single Cell V(D)J libraries incorporate standard Illumina paired-end constructs with P5 and P7 sequences at opposite ends. The 16bp 10x Barcode and the 10bp UMI are located at the start of Read 1, while sample index sequence information is incorporated into the i7 index read. Read 1 and Read 2 are standard Illumina sequencing primer sites used in paired-end sequencing.

FOR USE WITH

• Chromium™ Single Cell 5’ Library & Gel Bead Kit, 16 rxns PN-1000006
• Chromium™ Single Cell V(D)J Enrichment Kit, Human T Cell, 96 rxns PN-1000005
• Chromium™ Single Cell A Chip Kit, 48 rxns PN-120236
• Chromium™ Single Cell A Chip Kit, 16 rxns PN-1000009
• Chromium™ i7 Multiplex Kit, 96 rxns PN-120262