1:1 mixture of human (GM12878) and mouse (A20) cells. This is a classic human-mouse mixture experiment to demonstrate single cell behavior.
Libraries were prepared following the Chromium Next GEM Single Cell ATAC Reagent Kits v1.1 (User Guide CG000209 Rev A). All GM12878:A20 mixture libraries (v1.1 chemistry vs. v1 chemistry at all cell loads) were prepared with the same nuclei preparation.
Please consult the Cell Ranger ATAC 2.0.0 release notes for changes to the ATAC reference that may impact the interpretation of outputs in comparison to Cell Ranger ATAC 1.2.0.
- ~1,500 transposed nuclei were loaded. ~1,000 expected nuclei recovered. 515 human and 495 mouse nuclei were detected.
- Sequenced on Illumina NovaSeq with approximately 38k read pairs per cell.
- 50 bp Read 1
- 8 bp i7 index (sample index)
- 16 bp i5 (10x Barcode)
- 49 bp Read 2