10k 1:1 Mixture of Human GM12878 and Mouse EL4 cells, ATAC v1.1, Chromium X

Single Cell ATAC dataset analyzed using Cell Ranger ATAC 2.0.0

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Dataset overview

Output and supplemental files

Input files

Learn about Chromium analysis

1:1 mixture of human (GM12878, Coriell Institute) and mouse (EL4, ATCC) cells obtained by 10x Genomics were used in a classic experiment to demonstrate single cell behavior. Nuclei were isolated using the demonstrated protocol Nuclei Isolation for Single Cell ATAC Sequencing (CG000169).

ATAC libraries were generated from ~15,400 transposed nuclei (4,689 human nuclei and 4,891 mouse nuclei recovered) as described in the Chromium Single Cell ATAC Reagent Kits User Guide (v1.1 Chemistry) (CG000209 Rev E) using the Chromium X and sequenced on an Illumina NovaSeq 6000 to approximately 50k read pairs per cell.

50 cycles Read 1, 8 cycles i7 (sample index), 16 cycles i5 (10x barcode), 49 cycles Read 2.

This dataset is licensed under the Creative Commons Attribution license.