10k 1:1 Mixture of Human HEK293T and Mouse NIH3T3 Cells, Chromium GEM-X Single Cell 3'
Single Cell Gene Expression dataset analyzed using Cell Ranger 8.0.0
Assess data quality
View summary metrics to assess data quality and more.
Visualize and explore data
Discover differentially expressed genes, visualize your favorite genes, and explore your data with our visualization software.
Dataset overview
Output and supplemental files
Input files
Learn about Chromium analysis
A 1:1 mixture of fresh frozen human HEK293T and mouse NIH3T3 cells, obtained by 10x Genomics from ATCC, was used in a classic experiment to demonstrate single cell behavior.
Gene Expression libraries were generated as described in the Chromium GEM-X Single Cell 3' Reagent Kits v4 User Guide (CG000731) and sequenced on an Illumina NovaSeq 6000 with approximately 50,000 read pairs per cell.
Paired-end, dual indexing:
- 28 cycles Read 1
- 10 cycles i7
- 10 cycles i5
- 90 cycles Read 2
The cellranger multi
pipeline in Cell Ranger v8.0.0 was used to analyze libraries.
This dataset is licensed under the Creative Commons Attribution license.