Cryopreserved human peripheral blood mononuclear cells (PBMCs) from a healthy donor obtained by 10x Genomics from AllCells were used to demonstrate single cell behavior in blood. Nuclei were isolated using the demonstrated protocol Nuclei Isolation for Single Cell ATAC Sequencing (CG000169).
ATAC libraries were generated as described in the Chromium Single Cell ATAC Reagent Kits User Guide (v2 chemistry) (CG000496) using the Chromium X and sequenced on Illumina NovaSeq 6000 to approximately 45k read pairs per cell. 10,273 PBMC nuclei were recovered.
Paired-end, dual indexing:
- 50 cycles Read 1
- 8 cycles i7 (sample index)
- 16 cycles i5 (10x barcode)
- 49 cycles Read 2