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12k 1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells

Universal 3' dataset analyzed using Cell Ranger 1.2.0

Number of samples on this datasets page1

1:1 mixture of fresh frozen human (HEK293T) and mouse (NIH3T3) cells. This is a classic human-mouse mixture experiment to demonstrate single cell behavior.

  • 12,806 cells detected
  • Sequenced on Illumina Hiseq4000 with approximately 30,000 reads per cell
  • 26bp read1 (16bp Chromium barcode and 10bp UMI), 98bp read2 (transcript), and 8bp I7 sample barcode
  • Analysis run with --cells=12000

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.

Product

Universal 3'

Software

Cell Ranger

Subpipeline

cellranger count

10x Instrument(s)

Chromium Controller

Species

Human, Mouse

Anatomical Entity

Embryonic Kidney, Embryo

Preservation Method

Fresh Frozen

Cells or Nuclei

Cells

Selected Cell Types

fibroblast

Biomaterial Type

Cell Line

Cell/Nuclei Count

12,806

Donor Count

2

Date Published

2017-02-09