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2k Sorted Cells from Human Glioblastoma Multiforme, 3’ v3.1

Universal 3' dataset analyzed using Cell Ranger 6.0.0

Number of samples on this datasets page1

Human Glioblastoma Multiforme cells from a male donor aged 71 were obtained by 10x Genomics from Discovery Life Sciences.

Libraries were generated following the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index) User Guide (CG000315) and sequenced on Illumina NovaSeq 6000.

Sequencing Depth: 72,259 mean reads per cell

Paired-end, dual indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI) i5 index: 10 cycles (sample index) i7 index: 10 cycles (sample index) Read 2: 90 cycles (transcript)

Analysis Parameters Used: Run with --expect-cells=2000

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.

Product

Universal 3'

Software

Cell Ranger

Subpipeline

cellranger count

10x Instrument(s)

Chromium Controller

Species

Human

Anatomical Entity

Brain

Disease State

glioblastoma multiforme

Cells or Nuclei

Cells

Selected Cell Types

Sorted

Biomaterial Type

Specimen from Organism

Cell/Nuclei Count

1,615

Donor Count

1

Date Published

2021-03-31