Peripheral blood mononuclear cells (PBMCs) from a healthy donor were stained with a panel of 31 TotalSeq™-B antibodies (BioLegend).
PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).
Libraries were prepared following the Chromium Single Cell 3ʹ Reagent Kits v3 with Feature Barcoding technology for Cell Surface Protein User Guide (CG000185 RevB).
- 5,247 cells detected
- Sequenced on Illumina NovaSeq with approximately 28,918 reads per cell
- 28 bp Read 1 (16 bp Chromium barcode and 12 bp UMI)
- 91 bp Read 2 (transcript)
- 8 bp i7 (sample barcode)
- run with
--expect-cells=5000