1:1 mixture of human (GM12878) and mouse (A20) cells. This is a classic human-mouse mixture experiment to demonstrate single cell behavior.
Libraries were prepared following the Chromium Next GEM Single Cell ATAC Reagent Kits v1.1 (User Guide CG000209 Rev A). All GM12878:A20 mixture libraries (v1.1 chemistry vs. v1 chemistry at all cell loads) were prepared with the same nuclei preparation.
- ~750 transposed nuclei were loaded. ~500 expected nuclei recovered. 251 human and 238 mouse nuclei were detected.
- Sequenced on Illumina NovaSeq with approximately 31k read pairs per cell:
- 50 bp Read 1
- 8 bp i7 index (sample index)
- 16 bp i5 (10x Barcode)
- 49 bp Read 2