Aggregated PBMCs from a CMV Seronegative Donor - 5' Gene Expression with a Panel of dCODE™ Dextramer® Reagents and TotalSeq™-C Antibodies
Single Cell Immune Profiling dataset analyzed using Cell Ranger 3.0.2
Assess data quality
View summary metrics to assess data quality and more.
Visualize and explore data
Discover differentially expressed genes, visualize your favorite genes, and explore your data with our visualization software.
Dataset overview
Output and supplemental files
Learn about Chromium analysis
Peripheral blood mononuclear cells (PBMCs) from a CMV seropositive donor, were obtained through a collaboration. Cells were stained with a panel of 12 dCODE™ Dextramer® reagents (Immudex) and 17 TotalSeq™-C antibodies (BioLegend). See details in the Application Note “Redefining Cellular Phenotyping: Comprehensive Characterization and Resolution of the Antigen-Specific T Cell Response”.
The gene expression and cell surface protein libraries from the sorted (vdj_v1_hs_sorted_cmvneg_5gex_protein) and unsorted (vdj_v1_hs_unsorted_cmvneg_5gex_protein) samples were aggregated using Cell Ranger aggr.
- 7,346 total cells detected
- 1,438 median genes per cell and 4,524 median UMI counts per cell detected
- ~53,000 read pairs per cell pre-normalization; ~49,000 reads per cell post-normalization
This dataset is licensed under the Creative Commons Attribution license.