Peripheral blood mononuclear cells (PBMCs) from a CMV seropositive donor, were obtained through a collaboration. Cells were stained with a panel of 12 dCODE™ Dextramer® reagents (Immudex) and 17 TotalSeq™-C antibodies (BioLegend). See details in the Application Note “Redefining Cellular Phenotyping: Comprehensive Characterization and Resolution of the Antigen-Specific T Cell Response”.
The gene expression and cell surface protein libraries from the sorted and unsorted samples were aggregated using Cell Ranger aggr.
8,549 total cells detected 1,303 median genes per cell and 4,431 median UMI counts per cell detected ~73,000 read pairs per cell pre-normalization; ~53,000 reads per cell post-normalization