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Integrated GEX and VDJ analysis of Connect generated library from human melanoma

Single Cell Immune Profiling dataset analyzed using Cell Ranger 6.0.1

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Dissociated tumor cells from a primary human melanoma sample were purchased from Discovery Life Sciences. Gene Expression, TCR and BCR libraries were generated from a single channel of Chromium Connect. Libraries were prepared following the Chromium Next GEM Automated Single Cell 5' Reagent Kits v2 User Guide (CG000384).

  • 5,000 cells targeted
  • 4,680 cells detected
  • 30,038 median reads per cell
  • Sequenced on Illumina NovaSeq with 90 bp read 1, 90 bp read 2, 10 bp i5 sample barcode, and 10 bp i7 sample barcode
  • Run with Cell Ranger 6.0.1

This dataset is licensed under the Creative Commons Attribution license.