Integrated GEX, TotalSeq™-C, and BCR Analysis of Chromium Connect Generated Library from 10k Human PBMCs
Single Cell Immune Profiling dataset analyzed using Cell Ranger 6.1.2
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Dataset overview
Output and supplemental files
Input files
Learn about Chromium analysis
Peripheral blood mononuclear cells (PBMCs) were isolated from a healthy donor. Gene Expression, BCR, and Antibody Capture libraries were generated from a single channel of Chromium Connect. Antibody libraries were generated using BioLegend TotalSeq™-C TBNK panel. Libraries were prepared following the Chromium Next GEM Automated Single Cell 5' Reagent Kits v2 User Guide (CG0000507).
- 10,000 cells targeted
- 11,075 cells detected
- 68,396 mean reads per cell
- Sequenced on Illumina NovaSeq with 28 bp read 1, 90 bp read 2, 10 bp i5 sample barcode, and 10 bp i7 sample barcode
- Run with Cell Ranger 6.1.2
This dataset is licensed under the Creative Commons Attribution license.