Integrated GEX, TotalSeq™-C, and BCR Analysis of Chromium Connect Generated Library from 10k Human PBMCs

Single Cell Immune Profiling dataset analyzed using Cell Ranger 6.1.2

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Dataset overview

Output and supplemental files

Input files

Learn about Chromium analysis

Peripheral blood mononuclear cells (PBMCs) were isolated from a healthy donor. Gene Expression, BCR, and Antibody Capture libraries were generated from a single channel of Chromium Connect. Antibody libraries were generated using BioLegend TotalSeq™-C TBNK panel. Libraries were prepared following the Chromium Next GEM Automated Single Cell 5' Reagent Kits v2 User Guide (CG0000507).

10,000 cells targeted
11,075 cells detected
68,396 mean reads per cell
Sequenced on Illumina NovaSeq with 28 bp read 1, 90 bp read 2, 10 bp i5 sample barcode, and 10 bp i7 sample barcode
Run with Cell Ranger 6.1.2

This dataset is licensed under the Creative Commons Attribution license.