Integrated GEX, TotalSeq™-C, and TCR Analysis of Chromium Connect Generated Library from 5k CMV+ T cells
Single Cell Immune Profiling dataset analyzed using Cell Ranger 6.1.2
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Dataset overview
Output and supplemental files
Input files
Learn about Chromium analysis
Human T cells were isolated from a patient who was previously diagnosed with cytomegalovirus (lot 5147JN21). Gene Expression, TCR, and Antibody Capture libraries were generated from a single Chromium Connect channel. Antibody libraries were generated using a combination of a BioLegend TotalSeq™-C TBNK panel, a CMV dextramer, and a non-binding control dextramer. The TCR library was downsampled to 5,000 reads per cell. Libraries were prepared following the Chromium Next GEM Automated Single Cell 5' Reagent Kits v2 User Guide (CG0000507).
- 5,000 cells targeted
- 5,268 cells detected
- 66,175 mean reads per cell
- Sequenced on Illumina NovaSeq with 28 bp read 1, 90 bp read 2, 10 bp i5 sample barcode, and 10 bp i7 sample barcode
- Run with Cell Ranger 6.1.2
This dataset is licensed under the Creative Commons Attribution license.