Biomaterials
Amyotrophic Lateral Sclerosis (ALS) mouse models containing the SOD1-G93A mutation and their non-carrier littermates were used in this study. Flash frozen mouse lumbar spinal cords were obtained from Charles River. 30 day old mice are considered presymptomatic, 63 day old mice display the onset of locomotor disease, and 112 day old mice display motor neuron death during the end stage. The included datasets are derived from 112 day old (P112) mice.
Sample preparation
Nuclei were isolated from 10-30mg of the mouse lumbar spinal cord using the Chromium Nuclei Isolation Kit following the protocol for Single Cell Gene Expression (CG000505).
Assay workflow
Libraries were generated as described in the Chromium GEM-X Single Cell 3' Reagent Kits v4 User Guide (CG000731).
Sequencing
- Sequencing instrument: Illumina NovaSeq X+
- Sequencing configuration: 28 bp read 1, 90 bp read 2, 10 bp i7 sample index, and 10 bp i5 sample index
- Sequencing depth: approximately 30k reads per cell
Analysis
Cell Ranger v10.0 was used to map FASTQ files to the reference, perform cell-calling, and output feature-barcode matrices for further analysis. This dataset represents six independent cellranger multi runs.
How to view data
To get started, download Loupe Browser v9.0 to explore the Loupe file, or read more about the other Cell Ranger outputs.