NSCLC tumor - Ig enrichment from amplified cDNA

Targeted enrichment of B cell Igs from a fresh surgical resection of a squamous non-small cell lung carcinoma tumor.

The same cDNA was used to generate gene expression (vdj_v1_hs_nsclc_5gex) and TCR (vdj_v1_hs_nsclc_t) specific libraries.

Libraries were prepared following the Single Cell V(D)J Reagent Kits User Guide (CG000086 Rev C).

~15,000 cells loaded
2,927 expected Ig-expressing cells recovered (based on CD79A/CD79B expressing fraction in the gene expression data)
2,993 cells detected with either single IgH or IgL/IgK or both
1,560 cells with productive V-J spanning (IgH, IgL/IgK) pair
Sequenced on an Illumina NovaSeq 6000 with NovaSeq software v1.2 with ~14,000 read pairs per cell
150bp read1 (including 16bp Chromium barcode, 10bp UMI, 13bp switch oligo and transcript), 150bp read2 (transcript), and 8bp i7 sample barcode read configuration

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