PBMCs of a Healthy Donor - 5' Gene Expression with a Panel of TotalSeq™-C Antibodies

Universal 5' dataset analyzed using Cell Ranger 3.0.0

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Dataset overview

Output and supplemental files

Input files

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Peripheral blood mononuclear cells (PBMCs) of a healthy donor, purchased from AllCells (Catalog #: PB001), 87% viable by Trypan blue stain. PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).

The same cDNA was used to generate TCR (vdj_v1_hs_pbmc2_t) and B cell Ig (vdj_v1_hs_pbmc2_b) specific libraries.

Libraries were prepared following the Single Cell V(D)J Reagent Kits User Guide (CG000186 RevA).

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.