C57Bl/6 mouse splenocytes were obtained by 10x Genomics from C&M Lab Pro.
Gene Expression, BCR Enriched, and TCR Enriched libraries were generated from 1,612 cells (87% viable, expected cell recovery 842) as described in the Chromium Next GEM Single Cell V(D)J Reagent Kits v1.1 (CG000207 Rev E) and sequenced on an Illumina NovaSeq 6000.
Sequencing Configuration:
- Gene Expression: Paired-end (26X91), single-indexed sequencing. Read 1: 26 cycles, i7 index: 8 cycles, i5 index: 0 cycles, Read 2: 91 cycles
- BCR Enriched: Paired-end (26X91), single-indexed sequencing. Read 1: 26 cycles, i7 index: 8 cycles, i5 index: 0 cycles, Read 2: 91 cycles
- TCR Enriched: Paired-end (26X91), single-indexed sequencing. Read 1: 26 cycles, i7 index: 8 cycles, i5 index: 0 cycles, Read 2: 91 cycles
Analysis Settings:
- Gene Expression: --expect-cells=1000