Splenocytes from C57BL/6 mice, 1k cells (v2)
Single Cell Immune Profiling dataset analyzed using Cell Ranger 4.0.0
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Dataset overview
Output and supplemental files
Input files
Learn about Chromium analysis
C57Bl/6 mouse splenocytes were obtained by 10x Genomics from C&M Lab Pro.
Gene Expression, BCR Enriched, and TCR Enriched libraries were generated from 1,612 cells (87% viable, expected cell recovery 842) as described in the Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) User Guide (CG000331 Rev A) and sequenced on an Illumina NovaSeq 6000.
Sequencing Configuration:
- Gene Expression: Paired-end (26X90), dual-indexed sequencing. Read 1: 26 cycles, i7 index: 10 cycles, i5 index: 10 cycles, Read 2: 90 cycles
- BCR Enriched: Paired-end (28X90), dual-indexed sequencing. Read 1: 28 cycles, i7 index: 10 cycles, i5 index: 10 cycles, Read 2: 90 cycles. Note: Read 1 requirement is 26 bp; 28 bp Read 1 is trimmed by Cell Ranger pipeline.
- TCR Enriched: Paired-end (26X90), dual-indexed sequencing. Read 1: 26 cycles, i7 index: 10 cycles, i5 index: 10 cycles, Read 2: 90 cycles
Analysis Settings:
- Gene Expression: --expect-cells=1000
This dataset is licensed under the Creative Commons Attribution license.