Peripheral blood mononuclear cells (PBMCs) from a CMV seropositive donor, were obtained through a collaboration. Cells were stained with a panel of 12 dCODE™ Dextramer® reagents (Immudex) and 17 TotalSeq™-C antibodies (BioLegend). See details in the Application Note “Redefining Cellular Phenotyping: Comprehensive Characterization and Resolution of the Antigen-Specific T Cell Response”.
Cells were 81% viable by Trypan blue stain.
The same cDNA was used to generate TCR (vdj_v1_hs_unsorted_cmvpos_t) specific libraries.
Libraries were prepared following the Single Cell V(D)J Reagent Kits User Guide (CG000186 RevA).