HashMax™-5 Cell Hashing Antibodies

MultiPro®

HashMax™-5 Cell Hashing Antibodies

The MultiPro HashMax-5 Cell Hashing DNA-barcoded antibody kits offer a cost-effective solution for single cell sequencing, while significantly enhancing experimental flexibility and performance. This advanced approach improves cell recovery rates, reduces undetected multiplet rates, and enables effective hashing across diverse sample types. The kits are available as two separate sets, each containing 4 unique HashMax-5 hashcodes, or as a complete set of all 8 hashcodes (10 reactions per hashcode). Fully validated for use with our Universal 5' and Flex assays, HashMax-5 ensures robust, high-quality single cell analysis with unparalleled precision and reliability.

Validation data gallery

Left panel: Human PBMCs stained with MultiPro HashMax-5 antibodies and processed through the Universal 5' workflow. Image shows successful antibody-based demultiplexing. 4 unique donors were used, each donor was split into two samples and stained with cell hashing antibodies.
Right Panel: Demultiplexing of human PBMCs based on SNP profiles. Each donor is represented by two clusters.

Left Panel: Cells from various samples were stained with MultiPro HashMax-5 antibodies and processed through the Universal 5' workflow. Image shows successful antibody-based demultiplexing. CD34+ bone marrow cells were included in this analysis.
Right Panel: Expression of CD34 gene, demonstrating successful staining of progenitor cells.

Differentiated iPSCs stained with MultiPro HashMax-5 antibodies and processed through the Flex workflow.

Product information

The MultiPro HashMax-5 Antibodies include a mix of anti-CD29 (TS2/16) and anti-B2M (1H3F4), designed to enable effective cell hashing across a wide range of primary cell types and cell lines.

Tested reactivity

Human

Host

Mouse IgG1

Class

Monoclonal

Type

Antibody

Storage buffer

Glycerol buffer

Storage conditions

Store at –20°C.

Recommended dilution

2 μL/sample

Component & size

HashKit 1 hashcodes A–D

Component

MultiPro HashMax-5, Hashcode A

MultiPro HashMax-5, Hashcode B

MultiPro HashMax-5, Hashcode C

MultiPro HashMax-5, Hashcode D

Size

G900123-10RXNS

G900124-10RXNS

G900125-10RXNS

G900126-10RXNS

HashKit 2 hashcodes E–H

Component

MultiPro HashMax-5, Hashcode E

MultiPro HashMax-5, Hashcode F

MultiPro HashMax-5, Hashcode G

MultiPro HashMax-5, Hashcode H

Size

G900127-10RXNS

G900128-10RXNS

G900129-10RXNS

G900130-10RXNS

HashKit 3 hashcodes A–H

Component

MultiPro HashMax-5, Hashcode A

MultiPro HashMax-5, Hashcode B

MultiPro HashMax-5, Hashcode C

MultiPro HashMax-5, Hashcode D

MultiPro HashMax-5, Hashcode E

MultiPro HashMax-5, Hashcode F

MultiPro HashMax-5, Hashcode G

MultiPro HashMax-5, Hashcode H

Size

G900123-10RXNS

G900124-10RXNS

G900125-10RXNS

G900126-10RXNS

G900127-10RXNS

G900128-10RXNS

G900129-10RXNS

G900130-10RXNS

Targets

CD29 (TS2/16), B2M (1H3F4)

Documentation

Flex protocol

Cell Hashing Protocol for Flex

Universal 5’ protocol

Cell Hashing Protocol for 5' Gene Expression

SDS

Background information

MultiPro HashMax-5 Antibodies offer versatile applications, including increasing target cell recovery, reducing the undetected multiplet rate, and enabling efficient analysis of limited cell samples when used with Flex and Universal 5’ profiling. Unlike other sample multiplexing solutions on the market, HashMax antibodies provide the unique advantage of independent hashtag library generation, offering enhanced control over sequencing outputs and significant improvements in overall data quality.