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1:1 Mixture of Fresh Frozen Human (HEK293T) and Mouse (NIH3T3) Cells

Universal 3' dataset analyzed using Cell Ranger 1.1.0

Number of samples on this datasets page1

1:1 mixture of fresh frozen human (HEK293T) and mouse (NIH3T3) cells.

This is a classic human-mouse mixture experiment to demonstrate single cell behavior.

  • 1,387 cells detected
  • Sequenced on Illumina NextSeq 500 with approximately 80,000 reads per cell
  • 98bp read1 (transcript), 8bp I5 sample barcode, 14bp I7 GemCode barcode and 10bp read2 (UMI)
  • Analysis run with --cells=1200

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.

Product

Universal 3'

Software

Cell Ranger

Subpipeline

cellranger run

Species

Mouse, Human

Anatomical Entity

Embryonic Kidney, Embryo

Preservation Method

Fresh Frozen

Cells or Nuclei

Cells

Selected Cell Types

fibroblast

Biomaterial Type

Cell Line

Cell/Nuclei Count

1,387

Donor Count

2

Date Published

2016-05-26