Splenocytes from a C57BL/6 mouse strain were obtained by 10x Genomics from BioIVT.
Gene Expression, VDJ-T, and VDJ-B libraries were generated as described in the Chromium GEM-X Single Cell 5' Reagent Kits v3 User Guide (CG000733) and sequenced on an Illumina NovaSeq 6000 with approximately 37,000 read pairs per cell for the Gene Expression library, 30,000 read pairs per cell for the VDJ-T library and 14,000 read pairs for the VDJ-B library.
Paired-end, dual indexing libraries were sequenced with the following configuration:
- 28 cycles for Read 1
- 10 cycles for i7
- 10 cycles for i5
- 90 cycles for Read 2
Libraries were analyzed using the cellranger multi pipeline.
See companion datasets