10x Genomics Support/Single Cell ATAC/Documentation/Library Construction/

Workflow Update for Chromium Next GEM Chip and Gasket Assembly

Learn more about a change in the recommended chip assembly and loading technique

What do I need to know?

  • 10x Genomics is committed to providing customers the best experience to support their research needs.
  • We have observed that, on occasion, the gasket may misalign to our Chromium chips when assembling per our current workflow guidance for the assays listed below.
  • The misalignment most often results in a pressure error occurring early in the run on the Chromium X/iX or Chromium Controller. If this occurs, it can usually be resolved by re-opening and closing the secondary holder lid to allow the gasket to re-align with the wells on the chip.
  • To reduce the risk of gasket misalignment, we recommend assembling the gasket onto the secondary holder before inserting the chip and loading the reagents.
  • This change of workflow will not have an impact on the experiment or data.
  • Link to Customer Notification Letter here

Please reference the following affected assays:

  • Chromium Single Cell Gene Expression (LT and Standard Throughput)
  • Chromium Single Cell Immune Profiling (Standard Throughput)
  • Chromium Single Cell Multiome ATAC + Gene Expression
  • Chromium Single Cell ATAC
  • Chromium Fixed RNA Profiling

List of Chromium chip impacted

Part numberProduct name
1000143Chromium Next GEM Training Reagents, Gel Beads and Chip Kit
1000120Chromium Next GEM Chip G Single Cell Kit, 48 rxns
1000127Chromium Next GEM Chip G Single Cell Kit, 16 rxns
1000422Chromium Next GEM Chip Q Single Cell Kit, 16 rxns
1000418Chromium Next GEM Chip Q Single Cell Kit, 48 rxns
1000161Chromium Next GEM Chip H Single Cell Kit, 48 rxns
1000162Chromium Next GEM Chip H Single Cell Kit, 16 rxns
1000230Chromium Next GEM Chip J Single Cell Kit, 16 rxns
1000234Chromium Next GEM Chip J Single Cell Kit, 48 rxns
1000286Chromium Next GEM Chip K Single Cell Kit, 48 rxns
1000287Chromium Next GEM Chip K Single Cell Kit, 16 rxns
1000321Chromium Next GEM Chip L Single Cell Kit, 16 rxns

New How-To Video Available

Please click HERE to view a video demonstration of the updated technique and step-by-step guidance.

Document Type
Product Notification

Last Modified
August 4, 2022