10x Genomics Support/Single Cell Gene Expression Flex/Documentation/Sequencing/

Interpreting Cell Ranger multi Web Summary Files for Fixed RNA Profiling

  • CG000729_TechNote_WebSummary_Chromium_FixedRNAProfiling_RevA.pdf

    Technical Note, CG000729

    CG000729_TechNote_WebSummary_Chromium_FixedRNAProfiling_RevA.pdf

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The web summary file (web_summary. html) output by the cellranger multi pipeline is the initial point of reference for determining sample performance in the Chromium Fixed RNA Profiling (Single Cell Gene Expression Flex) assay. This Technical Note presents an overview of web summary file interpretation, including the expected metrics and characteristic plots for Gene Expression libraries generated using this Fixed RNA Profiling (FRP) assay. Metrics associated with product extensions (i.e., Feature Barcode technology) are not included in this document.

Document Type
Technical Note

Last Modified
January 31, 2024