Flex Gene Expression
Workflow Overview
Experimental Design & Planning
Protocol Planner
provides workflow overviews, document resources, reagents, and consumables for the GEM-X Flex v2 protocols.
Sample Preparation
Fixation of Cells & Nuclei for GEM-X Flex Gene Expression
Outlines how to perform fixation on single cell and nuclei suspensions for GEM-X Flex Gene Expression.
Tissue Fixation & Dissociation for GEM-X Flex Gene Expression
Provides step-by-step guidance on how to perform tissue fixation and dissociation for GEM-X Flex Gene Expression
Sample Preparation from FFPE Tissue Sections for GEM-X Flex Gene Expression
Provides step-by-step guidance on how to dissociate FFPE samples for GEM-X Flex Gene Expression protocols.
Blood Fixation and Cell Isolation for GEM-X Flex Gene Expression
This protocol outlines fixation of blood and isolation of peripheral blood mononuclear cells (PBMCs) and leukocytes from the fixed blood for use with the GEM-X Flex Gene Expression.
Plate-based Sample Preparation for GEM-X Flex v2
Provides sample preparation protocols in a 96-well v-bottom deep-well plate to enable high throughput handling of up to 384 samples for use with GEM-X Flex v2.
Library Construction
GEM-X Flex v2 For Multiplexed Samples
Provides instructions for constructing Multiplexed Gene Expression libraries.
GEM-X Flex v2 For Singleplexed Samples with optional Feature Barcode technology for Protein
Provides instructions for constructing Singleplex Gene Expression libraries or Gene and Protein Expression libraries.
Sequencing
Sequencing Requirements for Flex Gene Expression
Provides sequencing requirements for Fixed RNA Profiling libraries.
Data analysis
Cell Ranger
Analyze single cell gene expression data with a set of free, easy-to-use analysis pipelines.
Pipeline Runner
Formerly known as Cloud Analysis. Analyze 10x Genomics data quickly and securely in the cloud.
Sentira Single Cell
Analyze Chromium single cell data through interactive, agentic workflows.
Loupe Browser
Explore 10x Genomics data with our powerful visualization software. Compatible with single cell and spatial products.
How-to videos
Other resources
Knowledge base Q&A
Find answers to common technical questions, from sample prep through data analysis, about the Flex Gene Expression workflow.
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Datasets
Access free, publicly available data from a number of different sample types generated with Chromium single cell solutions.
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Publications
Read key peer-reviewed research publications offering greater insights into biology, enabled by 10x Genomics products and technologies.
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